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Predicted I Observed M.W.: 128 kDa                                                          Uniprot ID: Q9LK10

Application: WB

Quantity:        50 ul CHR40 (M) (R2156-2b) Rabbit Polyclonal Antibody

                      50 ul CHR40 (C) (R2156-3) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Chromatin remodeling 40 (CHR40) is a nuclear protein that possesses helicase activity. CHR40 has been implicated in DNA binding, ATP binding, and nucleic acid binding. CHR40 may be involved in cell-cell signaling, histone lysine methylation, production of miRNAs involved in gene silencing by miRNA, production of ta-siRNAs involved in RNA interference, regulation of flower development, and virus induced gene silencing.

Other Names:

Chromatin remodeling 40, Genomic DNA, chromosome 3, TAC clone: K7M2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle [CHR40 (M) (R2156-2b)] or the C-terminal [CHR40 (C) (R2156-3)] region of arabidopsis thaliana CHR40 (At3g24340). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: