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Predicted I Observed M.W.: 78 I 100 kDa                                                   Uniprot ID: O43164

Application: WB

Quantity:        50 ul PJA2 (N) (R2132-1) Rabbit Polyclonal Antibody &

                        50 ul PJA2 (C) (R2132-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

E3 ubiquitin-protein ligase Praja-2 (PJA2) contains a RING-type zinc finger domain and has E2-dependent E3 ubiquitin-protein ligase activity. PJA2 is responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. PJA2 is essential for PKA-mediated long-term memory processes.

Other Names:

E3 ubiquitin-protein ligase Praja-2, Praja2, RING finger protein 131, KIAA0438, Neurodap1, RNF131

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [PJA2 (N) (R2132-1)] or the C-terminal [PJA2 (C) (R2132-2)]  region of human PJA2.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: