Products

R0095-vp - FLT3L (vPairTM) Antibodies

Catalog No. R0095-vp
Product Name FLT3L (vPairTM) Antibodies
Price $249
  
 

Predicted M.W.: 26 kDa                                                        Uniprot ID: P49772

Application: WB, ELISA

Quantity:       50 ug FLT3L (C) (R0095-1) Rabbit Polyclonal Antibody &

                     50 ug FLT3L (N) (R0095-2) Rabbit Polyclonal Antibody


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Fms-related tyrosine kinase 3 ligand (FLT3L) is a cytokine that stimulates the proliferation of early hematopoietic cells.  It synergizes with a number of other colony stimulating factors and interleukins.  Structurally it is similar to stem cell factor and colony stimulating factor 1.             

Other Names:

Flt3lg, Ly72L

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the C-terminal region [FLT3L(C), R0095-1], or the N-terminal region [FLT3L(N), R0095-2] of mouse FLT3L.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect recombinant protein)

ELISA: 1:1,000- 1:10,000 (both antibodies detect native recombinant protein in a direct ELISA assay). Suitable for using as ELISA capture or detection antibody.

Product Data:

FLT3L Antibody Western (Abiocode)

Fig 1. Western blot of recombinant mouse
FLT3L protein (A&C: 10 ng; B&D: 100 ng),
using indicated Abs at RT for 2 h.