Products

R2805-vp - RXFP1 (vPairTM) Antibodies

Catalog No. R2805-vp
Product Name RXFP1 (vPairTM) Antibodies
Price $299
  
 

Predicted I Observed M.W.: 87 I 110 kDa                                                   Uniprot ID: Q9HBX9

Application: WB, IP

Quantity:        50 ul RXFP1 (N) (R2805-1) Rabbit Polyclonal Antibody &

                      50 ul RXFP1 (C1) (R2805-2) Rabbit Polyclonal Antibody 


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Relaxin family peptide receptor 1 (RXFP1) is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane receptor superfamily. RXFP1 plays a critical role in sperm motility, pregnancy and parturition as a receptor for the protein hormone relaxin. Decreased expression of the RXFP1 gene may play a role in endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq].

Other Names:

Relaxin receptor 1, Leucine-rich repeat-containing G-protein coupled receptor 7, Relaxin family peptide receptor 1, LGR7, RXFPR1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [RXFP1 (N) (R2805-1)] or the C-terminal [RXFP1 (C1) (R2805-2)] region of human RXFP1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1. A) Western blot of total cell extracts from a,d. human HeLa, c. mouse brain,
b,e. human Jurkat; using 2 independent Abs against 2 distinct regions of human
RXFP1 at RT for 2 h. B) Total extracts from human HeLa were immunoprecipitated
with IgG or anti-RXFP1 (C1) (R2805-2); followed by WB with the same Ab.