Products

R3139-vp - TOC1 (vPairTM) Antibodies

Catalog No. R3139-vp
Product Name TOC1 (vPairTM) Antibodies
Price $379
  
 

Predicted I Observed M.W.: 69 I 75 kDa                                                     Uniprot ID: Q9LKL2

Application: WB

Quantity:        50 ul TOC1 (N) (R3139-1) Rabbit Polyclonal Antibody &

                        50 ul TOC1 (C) (R3139-2) Rabbit Polyclonal Antibody 


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

TOC1 is a Pseudo response regulator involved in the generation of circadian rhythms. TOC1 appears to shorten the period of circumnutation speed. TOC1 contributes to the plant fitness (carbon fixation, biomass) by influencing the circadian clock period. PRR3 may increase the stability of TOC1 by preventing interactions between TOC1 and the F-box protein ZTL. Expression of TOC1 is correlated with rhythmic changes in chromatin organization [provided by TAIR].

Other Names:

Two-component response regulator-like APRR1, ABI3-interacting protein 1, Pseudo-response regulator 1, Timing of CAB expression 1, APRR1, ATTOC1, PRR1, PSEUDO-RESPONSE REGULATOR 1, TIMING OF CAB EXPRESSION 1, AIP1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [TOC1 (N) (R3139-1)] or the C-terminal [TOC1 (C) (R3139-2)] region of arabidopsis thaliana TOC1 (At5g61380). Antibodies were purified by affinity purification using immunogen.

Species Specificity:

Arabidopsis thaliana

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

TOC1 Antibody Western (Abiocode)

Fig 1. Western blot of equal amounts of protein extracts
from WT or TOC1 deficient mutant (Mut) arabidopsis; using
2 independent Abs against 2 distince regions of arabidopsis
TOC1 (At5g61380) at RT for 2 h. The same filters were
re-probed with a non-relevant Ab for loading controls.