Products

R0031-vp - HELLS (vPairTM) Antibodies

Catalog No. R0031-vp
Product Name HELLS (vPairTM) Antibodies
Price $249
  
 

Predicted M.W.: 97 kDa                                                                    Uniprot ID: Q9NRZ9

Application: WB

Quantity:        50 ul HELLS (N) (R0031-1) Rabbit Polyclonal Antibody &

                      50 ul HELLS (C) (R0031-2) Rabbit Polyclonal Antibody


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Lymphoid-specific helicase (HELLS) plays an essential role in normal development and cell survival.  It is involved in the regulation of the expansion and survival of lymphoid cells.  HELLS is required for de novo and maintenance of DNA methylation.

Other Names:

LSH, PASG, SMARCA6, FLJ10339, Nbla10143

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [HELLS (N) (R0031-1)] or the C-terminal region [HELLS (C) of human HELLS. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

HELLS Antibody Western (Abiocode)

Fig 1.  Western blot of total cell extracts
from human HeLa, using Abs (A:R0031-1;
B: R0031-2) at RT for 2 h.