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Quantity:        50 ul SMARCA2 (N) (R0109-1) Rabbit Polyclonal Antibody &

                      50 ul SMARCA2 (C) (R0109-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily a, member 2 (SMARCA2) is a member of the SWI/SNF family of proteins.  It is a transcriptional co-activator cooperating with nuclear hormone receptors to potentiate transcriptional activation.  It is also involved in vitamin D-coupled transcription regulation.  SMARCA2 is similar to the Drosophila protein Brahma.                                                                            

Other Names:

Probable global transcription activator SNF2L2, ATP-dependent helicase SMARCA2, BRG1-associated factor 190B, BAF190B, BAF190, Protein brahma homolog, hBRM, SNF2-alpha, SNF2A, SNF2LA, SNF2L2, FLJ36757, MGC74511, SWI2, Sth1p, hSNF, BRM

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [SMARCA2 (N) (R0109-1)] or the C-terminal region [SMARCA2 (C) (R0109-2)] of human SMARCA2.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: