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Quantity: 100 ul                                                                                       Application: WB

Predicted I Observed M.W.: 36 I 36, 53, 90 kDa                                       Uniprot ID: Q9LN63

Background:

BRI1-EMS-SUPPRESSOR1 (BES1) is a brassinosteroid (BR) signalling protein that accumulates in the nucleus as dephosphorylated form in response to BRs. BES1 is phosphorylated by the BIN2 GSK3 kinase. It synergistically interacts with BIM1 to bind to E box sequences (CANNTG). The BES1  protein contains a nuclear localization signal (NLS), followed by a highly conserved amino-terminal domain (N) shared by all family members, a BIN2 phosphorylation domain (P), a PEST motif involved in protein degradation in the absence of BR, and a carboxyl-terminal domain. BES1 can interact with the ELF6 and REF6 Jumonji N/C-domain containing proteins and may direct them to modify histone methylation upstream of some brassinosteroid responsive-genes.

Other Names:

BRASSINAZOLE-RESISTANT 2, BRI1-EMS-SUPPRESSOR1, BZR2, Protein 107, Protein BIN2 SUBSTRATE 1, At1g19350, F18O14_4, BIS1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana BES1 (AT1G19350). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: