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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 128 kDa                                                          Uniprot ID: Q9SQI2

Background:

Protein GIGANTEA (GI) is involved in regulation of circadian rhythm and photoperiodic flowering, and may play a role in the maintenance of circadian amplitude and period length. GI is involved in phytochrome B signaling.  GI stabilizes ADO3 and the circadian photoreceptor ADO1/ZTL, and regulates 'CONSTANS' (CO) in the long-day flowering pathway by modulating the ADO3-dependent protein stability of CDF1 and CDF2, but is not essential to activate CO transcription. GI regulates, via the microRNA miR172, a CO-independent pathway that promotes photoperiodic flowering by inducing 'FLOWERING LOCUS T'. 

Other Names:

Protein GIGANTEA

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana GI (At1g22770). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: