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R1244-2 - PHYB (C1) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB
Predicted I Observed M.W.: 129 kDa Uniprot ID: P14713
Background:
Phytochrome B (PHYB) is a regulatory photoreceptor, which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion. PHYA is involved in the flowering time regulation.
Other Names:
Phytochrome B, HY3
Source and Purity:
Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana PHYB (At2g18790). Antibodies were purified by affinity purification using immunogen.
Storage Buffer and Condition:
Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.
Species Specificity:
Arabidopsis thaliana
Tested Applications:
WB: 1:500-1:2,000 (detect endogenous protein*)
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data:
Fig 1. A) Western blot analysis of total protein extracts from wild type (WT) or Myc-tagged PHYB overexpressing (Myc-PHYB) arabidopsis leaves, using anti-PHYB (C1) (R1244-2) at RT for 2 h. B) Same as in A except that protein extracts(100ug) were prepared from 4 day-old, dark-grown WT or phyB-5 null mutant (Mut) arabidopsis seedlings, using the same Ab at 1:1000 dilution at 40C overnight (data in B provided by W. Hu, UC Davis).
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