Abiocode Logo

Predicted M.W.: 21 kDa                                                                                Uniprot ID: O88713

Application: WB

Quantity:        50 ul KLRG1 (M) (R0253-1) Rabbit Polyclonal Antibody &

                      50 ul KLRG1 (C) (R0253-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Killer cell lectin-like receptor subfamily G member 1 (KLRG1) is a single-pass type II cell membrane protein that is expressed specifically on natural killer (NK) cells and activated CD8 T-cells and is induced by pathogens and viral infections. KLRG1 forms monomer and disulfide-linked homodimer. KLRG1 plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. KLRG1 may mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.

Other Names:

Killer cell lectin-like receptor subfamily G member 1, Mast cell function-associated antigen 2F1, Mafa

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the middle [KLRG1 (M) (R0253-1)] or the C-terminal [KLRG1 (C) (R0253-2)] regions of mouse KLRG1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: