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Quantity: 100 ul                                                                                 Application: WB

Predicted M.W.: 38 kDa                                                                    Uniprot ID: P17947

Background:

Transcription factor PU.1, also known as SPI1, belongs to the ETS family. It binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. PU.1 is a transcriptional activator involved in the differentiation or activation of macrophages and B lymphocytes. PU.1 also binds to RNA and may modulate pre-mRNA splicing. PU.1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the Spi-1 gene due to the insertion of spleen focus-forming virus (SFFV).

Other Names:

SPI1, Transcription factor PU.1, 31 kDa-transforming protein

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human PU.1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:500-1:1,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: