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Predicted | Observed MW: 45 | 50 kDa                                           Uniprot ID: Q02962

Application: WB

Quantity:        50 ul PAX2 (M) (R0770-2) Rabbit Polyclonal Antibody &

                      50 ul PAX2 (C) (R0770-3) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Paired box protein Pax-2 is a probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. It interacts with ELGN3; the interaction targets PAX2 for destruction. Defects in PAX2 are the cause of renal-coloboma syndrome (RCS) and may be responsible for isolated renal hypoplasia.

Other Names:

Paired box protein Pax-2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the middle [PAX2 (M) (R0770-2)] or the C-terminal [PAX2 (C) (R0770-3)] region of human PAX2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: