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R1092-1 - TET3 (N1) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB, IP Predicted M.W.: 179 kDa Uniprot ID: O43151 Background: TET3 is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), and may play a role in DNA demethylation. TET3 is a member of the ten-eleven translocation (TET) gene family. Other Names: Methylcytosine dioxygenase TET3, KIAA0401 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human TET3. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:500-1:2,000 (detect endogenous protein*) Immunoprecipitation (IP): 1:50- 1:100 *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. (A) Western blot of total cell extracts from (a, c, e) human Jurkat, (b, d, f) mousethymus, using 3 independent Abs against 3 distinct regions of human TET3 at RT for 2 h. (B) IP-Western (WB). Total extracts from mouse thymus were immunoprecipitated with a. anti-TET2 (R1086-1a), b. anti-TET3 (R1092-1); followed by WB with monoclonal anti-TET3 (M1092-3) at RT for 2 h. |