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R2257-2 - PHC1 (C) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB, IP Predicted I Observed M.W.: 106 I 140 kDa Uniprot ID: P78364 Background: Polyhomeotic-like protein 1 (PHC1) is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Other Names: Polyhomeotic-like protein 1, hPH1, Early development regulatory protein 1, EDR1, PH1 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human PHC1. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) IP: 1:100-1:200
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. (A) Western blot of total cell extracts from a. human HeLa and b. human Jurkat, using anti-PHC1 (C) (R2257-2) at RT for 2 h. (B) Total extracts from human HeLa were immunoprecipitated (IP) with IgG or anti-PHC1 (C) (R2257-2); followed by WB with the same Ab at RT for 2 h. |