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R2819-1 - PMS2 (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB
Predicted I Observed M.W.: 96 kDa Uniprot ID: P54278 Background: Mismatch repair endonuclease PMS2 belongs to the DNA mismatch repair MutL/HexB family. PMS2 is a component of the post-replicative DNA mismatch repair system (MMR). PMS2 heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. Other Names: Mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 protein homolog 2, PMSL2 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human PMS2. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot of total cell extracts from (A) mouse brain, (B) mouse thymus, (C) human Jurkat, using anti-PMS2 (N) (R2819-1) at RT for 2 h. |