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R1515-2 - CDC25A (M) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB Predicted I Observed M.W.: 59 I 70 kDa Uniprot ID: P30304 Background: M-phase inducer phosphatase 1 (CDC25A), a Tyrosine protein phosphatase belonging to the MPI phosphatase family, functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDK1 and stimulates its kinase activity; CDC25A also dephosphorylates CDK2 in complex with cyclin E in vitro. Other Names: M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the middle region of human CDC25A. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot of total cell extractsfrom human Jurkat, anti-CDC25A (M) (R1515-2) at RT for 2 h. |