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Quantity: 100 ul                                                                                      Application: WB

Predicted I Observed M.W.: 53 I 57, 63 kDa                                            Uniprot ID: Q8GZM7

Background:

PIF1 is a transcription activator. PIF1 regulates negatively chlorophyll biosynthesis and seed germination in the dark, and light-induced degradation of PIF1 relieves this negative regulation to promote photomorphogenesis. PIF1 binds to the G-box motif (5'-CACGTG-3') found in many light-regulated promoters. PIF1 promotes the expression of SOM, and thus modulates responses to abscisic acid (ABA) and gibberellic acid (GA).

Other Names:

Transcription factor PIF1, Basic helix-loop-helix protein 15, AtbHLH15, bHLH 15, Protein PHY-INTERACTING FACTOR 1, Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 5, Transcription factor EN 101, bHLH transcription factor bHLH015, At2g20180, BHLH15, EN101, PIL5, T2G17.2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana PIF1 (AT2G20180). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: