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R1180-1 - SHC4 (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB, IP Predicted I Observed M.W.: 69 kDa Uniprot ID: Q6S5L8 Background: SHC4 activates both Ras-dependent and Ras-independent migratory pathways in melanomas. SHC4 contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1. SH4 is only expressed in melanomas. Other Names: SHCD Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human SHC4. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) IP: 1:100-1:200
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. (A) Western blot of total cell extracts from human Jurkat, using Ab (R1180-1) at RT for 2 h. (B) Total extracts from human Jurkat were immunoprecipitated (IP) with IgG or anti-SHC4 (N) (R1180-1); followed by WB with the same Ab at RT for 2 h. |